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Rajasthan Lab Technician Syllabus 2025 – Overview
| Recruiting Organization | Rajasthan State Institute of Health & Family Welfare (SIHFW) |
| Post Name | Lab Technician |
| Total Posts | 321 |
| Category | Syllabus & Exam Pattern |
| Selection Process |
Written Exam
Document Verification
Medical Examination
|
| State Hiring | Rajasthan |
| Offical Website | https://www.rajswasthya.nic.in/ |
Rajasthan Lab Technician Syllabus 2025
General Knowledge & General Science and Current Affairs :
1. History of India.
2. Indian National Movement with important events in Rajasthan.
3. Geography of India & Rajasthan.
4. Economy India & Rajasthan.
5. India & Rajasthan- State & Governance, Constitution, Political System, Panchayati Raj System.
6. Biodiversity in environmental conditions, climate change.
7. Main features of society of Rajasthan, social problem, culture of Rajasthan.
8. Current Events- India, Rajasthan & World.
9. General Science (10th standard)
General English :
1. Tenses
2. Rearranging of jumbled sentences
3. Narration (Direct & Indirect)
4. Modals
5. Articles & Determines
6. Comprehension with blanks to be filled in with
a Phrases
b. Pronouns
7. Homonyms/Homophones
8. Clauses
9. Synonyms and antonyms
10. Pairs of words and their use in meaningful sentences
11 Idioms and phrases
12. Uses of prepositions
13. Active and passive voice
General Hindi :
1. दिये गये शब्दों की संधि एवं वर संधिविच्छेदा
2. उपसर्ग एवं प्रत्ययसंरचना तथा शब्दों से उपसर्ग एवं इनके योग से शब्द प्रत्चय को पृथक करना, इनकी पहचान।
3. समस्त (सामासिक) पद की रचना करना, समस्त (सामासिक) पद का विग्रह काना।
4. शशष्ट सुरों का अर्थ भेद।
5. पर्यायवाची शब्द और विलोम शब्द ।
6. शब्द शुद्धि दिये गये अशुद्ध शब्दों को शुद्ध लिखना। –
7. वाक्य शुद्धि वर्तनी संबंधी अशुद्धियों को छोड़कर वाक्य संबंधी अन्य व्याकरणीय अशुद्धियो का शुद्धीकरण।
8. वाक्यांश के लिये एक उपयुक्त शब्द।
9. पारिभाषिक शब्दावलीन्धित अंग्रेजी शब्दों के समकक्ष हिन्दी प्रशासन से सम्ब शब्द।
10. मुहावरे एवं लोकोक्ति
Knowledge of Computers :
1. Basic Applications of computer and its component.
2. Fundamentals of computer sciences.
3. Concept of open source technologies.
4. Input & Output Devices.
5. Operating system
6. Knowledge of MS Word, MS Excel, MS Access, MS PowerPoint, PDF Internet and E-mail.
7. Concept of computer virus and anti-virus.
8. Hardware & software, elements of computers processing system Hardware, CPU, peripherals, storage media, software definition, role and categories firmware and human-ware.
9. Role of information technology in governance & IT Acts & Regulations.
National Health Programs :
1. Knowledge of National Health Mission, its various programs like RCH, Communicable and Non-Communicable Disease Control Programs and their activities.
2. Ayushman Bharat and related health programs.
Topic Related to Professional Knowledge :
1. Anatomy and Physiology
Anatomy and Physiology of the human body.
i. Cells: Structure and function
ii. Tissues: Epithelial, Muscular, Connective (Cartilage and bone) and necrosis.
iii. Blood
iv. Circulatory system
v. Digestive system
vi. Respiratory system Nervous system – Endocrine system
vii. Urinary system
viii. Ear, Nose, Tongue and skin.
ix. Skeletal system
x. Muscular system
xi. Reproductive system
2. Hematology and blood banking :-
a. Clinical Pathology and Quality Control
b. Clinical Pathology:-
i. Introduction to clinical pathology & safety measures in lab.
ii. Quality control – Internal and external.
iii. Urine analysis
iv. Collection, composition, preservation and gross examination of urine.
v. Chemical and microscopic examination of urine.
vi. Cerebrospinal fluid (CSF) examination.
vii. Examination of other body fluids.
viii. Semen analysis.
ix. Norms of biomedical waste and discarding of infected blood.
c. Hematology:
i. Introduction to clinical hematology, instruments and glassware’s
ii. Preparation of various stains, buffers and other solution used in hematology.
iii. Methods of collection of blood sample and anticoagulants used in different tests and various types of vacutioners.
iv. Red blood cell counting.
v. White blood cell count and absolute eosinophil count.
vi. Platelet and reticulocyte count.
vii. Methods of hemoglobin estimation, their merits and demerits.
viii. Packed cell volume.
ix. Blood indices.
x. Erythrocyte sedimentation rate.
xi. Preparation and staining of peripheral blood smear.
xii. Morphology of normal and abnormal forms of RBC’s.
xiii. Morphology of normal and abnormal forms of Leucocytes.
xiv. Differential Leucocyte count.
xv. Bone marrow examination – Different sites and needles used
xvi. Osmotic fragility test.
xvii. Estimation of foetal haemoglobin.
xviii. G6PD Estimation.
xix. Sickling test.
xx. Automation in Hematology – Basic principals
d. Blood Banking
i. Screening and selection of donor.
ii. Collection and storage of blood.
iii. Blood grouping – A B O RH, and other systems of blood groups, sub group of A, Bombay group, Antibodies to ABO System, Anti AB and Anti H antibody.
iv. ABO testing slides and tube test, reverse grouping, discrepancies between cell and serum results, sources of error, rouleux formation and methods of checking it.
v. RH grouping test slide or rapid tube test false – Positive and false – Negative results, Du system & its significance.
vi. Cross matching, reasons of cross match, saline, albumin, Coombs and enzymes in testing.
vii. Coombs test Directed and indirect, principle, explanation of procedure and sources of error control, interpretation and clinical application.
viii. Preparation of various components of the blood and their storage.
ix. Grouping and cross matching by Gel techniques.
e. Clinical pathology :-
x. Complete urine examination.
xi. Semen analysis.
xii. CSF and other body fluids examinations
xiii. Blood cell counts including reticulocyte count
xiv. Haemoglobin estimation, Haemoglobin electrophoresis.
xv. Determination of PC; ESR and blood indices.
xvi. L.E. cell test, test for cold agglutination.
xvii. Preparation and staining of peripheral blood and bone marrow smears.
xviii. Differential leucocyte count.
xix. Osmotic fragility test
xx. Sickling test
xxi. Foetal haemoglobin estimation
xxii. G6PD estimation.
xxiii. Blood grouping and cross matching
xxiv. Organization of blood bank, separation and uses of various components of blood.
xxv. Transfusion reactions.
3. Environmental studies:-
1.General
i) Biotic and a biotic environment
ii) Adverse effect of environmental pollution
iii) Control strategies
iv) Various acts and regulations.
II.Water Pollution
III.Air Pollution
IV.Land Pollution
4. Bio Medical waste management
i. Introduction to bio-medical waste
ii. Types of bio medical waste
iii. Collection of bio medical waste
iv. Treatment and safe disposal of bio-medical waste
5. Microbiology Including parasitological & immunology:-
a. Human Parasitology : Protozoa-classification, morphology life cycle (Diagrammatic) and lab diagnosis (short) E. Histolylica, giardia intestinalis, malarial parasite, trichomonas, leishmania, trypanosome, toxoplasma.
b. Nematodes : Classification, morphology life cycle (Diagrammatic) and lab diagnosis (Short) ascaris, ankylostoma duodenale, enterblus, vermicularis, trichuris, strongyloides, dracunculus, wuchereria, banacrofti, onchocerea.
c. Platyhelinthes :
i. Classification – Names with general outline, morphology, life cycle (Chart)
ii. Lab Diagnosis (Short)
iii. Teaenia solium
iv. Taenia saginata
v. Hymenolepis nana
vi. Echinococus granulosus
d. Systemic bacteriology
1. Morphology, culture, identification and laboratory diagn osis.
2. Gram positive cocci-staphylococci, streptococci, preumococci.
3. Gram negative cocci – Neisseria
4. Gram negative bacilli-Classification of enterobacteriraccae.
i. Morphology
ii. Culture
iii. Biochemical diagnosis.
iv. Laboratory diagnosis
v. Coli, klebsiella, Enterobacter, Proteus, Salmonella, Shigella, Vibrio Pseudomonas.
e. Fastidious
1. Haemoplilus
2. Gram positive bacilli
i. Morphology, culture, biochemical identification and lab diagnosis.
ii. Corynebacteriam.
iii. Clostridium
iv. Bacillus
f. Spirochactes – Morphology and serological (T. Pallidum diagnosis)
g. Mycobacteria – Morphology classification identification by biochemical tests.
6. Virology
1. General character with classification
2. Human immune deficiency virus
3. Hepatitis viruses.
7. Immunology : Antigen antibody reaction-Principles and practical application of various types of serological tests Precipitation, agglutination, complement fixation test, neutralization, ELISA, Radio immimo assasy, ceectrophoresis, immunofluorescence, polymerase chain reaction (in short), Hyper sensitivity-Definition and type only.
8. Pathology:-
a. Histopathology
i. General principles of histo-pathological work: Collection of specimen, nunberingand giving tissue bits.
ii. Equipments used in histopathology, their merits & denerits and care to be taken.
iii. Fixatives used in histopathology Preparation, advantage and disadvantages.
iv. Frozen section and cryostat technique, staining and mounting, morbid anatomy.
V. Decalcification Methods, advantages and disadvantages of each method
b. Cytology:
i. Introduction of cytopathology, meth ods of collection of materials making smears and preparations of fixatives used.
ii. Different stains used, their preparation and staining the smears.
iii. Demonstration of Barr- Bodies (Sex Chromatin).
c. Staining technique
i. Morbid anatomy
ii. Tissue processing – Fixation, dehydration, clearing impregnation in paraffin.
iii. Making of blocks and section cutting, errors in section cutting and their correction.
iv. Preparation of different types of haematoxylins and Eosins including staining technique of rapid diagnosis.
v. Haematoxylin and Eosin staining, including staining technique for rapid diagnosis and mounting (Different type of special stains)
vi. Preparation of different type of special stains
vii. Special staining techniques.
viii. Immuno-histochemical and immunio-cytochemical staining.
d. Hematology
i. Investigation of bleeding disorders- Normal coagulation cascade.
ii. Bleeding time and clotting time, methods and interpretation.
iii. Clot retractin time.
iv. Prothrombin time.
v. Activated partial thromboplastine time.
vi. Thrombin time
vii. Fibrinogen Degradation Products (FDP)
e. Mounting techniques
i. Preparation of specimen for mounting.
ii. Preparation of fixatives for mounting.
iii. Techniques of mounting
iv. Organization of medical laboratory and museum and their maintenance.
f. Bio-Chemistry:-
i. Digestion, absorption and assimilation of carbohydrates, blood sugar, regulation of blood sugar glycolysis, TCA, Cycle, glycogenesis, glycogenolysis, GTT, metabolic disease associated with carbohydrate metabolism, diabetes mellitus, glycated hemoglobin.
ii. Digestion, absorption of proteins, amino acids, urea formation, formation of creatinine, metabolic, disorders associated with amino acid metabolism, electrophoretic separation of proteins.
iii. Digestion and absorption and assimilation of lipids, ketone, bodies formation metabolic disorders associated with lipids, cholesterol and lipoprotein metabolism.
iv. Metabolic disorders associated with nucleic acid metabolism, gout etc.
v. Kidney function tests, urine formation, informal and abnormal constituents.
vi. Liver function tests and different type of jaundice.
vii. Cardiac profile tests application and significance.
viii. Pancreatic enzymes and diagnostic importance.
ix. Chemistry of blood, composition and importance of different constituents, hemoglobin biosynthesis, porphyrias and its composition and diagnostic significance.
x. Constituents of gastric juice and diagnostic importance.
xi. Principles of special investigation like RIA, ELISA and chemiluminescence.
xii. Normal values, normal range, interpretative clinical chemistry, quality controls internal and external.
xiii. Automation in clinical investigation Auto and semi analyzers, continuous flow analyzers, discrete analyzers, batch analyzers random access auto analyzer, dry chemistry analyzers – Reagents kits.
xiv. Recording of patients date, reporting values, preparation of investigation, statistics.
Rajasthan Lab Technician Exam Pattern 2025
Duration : 150 Minutes
Negative marking : 01
| S.No. | Subject | No.of Question | Marks |
| 1. | General Knowledge & General Science and Current Affairs | 15 | 45 |
| 2. | General English & Hindi | 15 | 45 |
| 3. | Knowledge of Computers | 15 | 45 |
| 4. | National Health Programs | 15 | 45 |
| 5. | Topic Related to Professional Knowledge | 90 | 270 |
| Total | 150 | 450 |
